Isolation and biological activity of frankiamide.
Identifieur interne : 002710 ( Main/Exploration ); précédent : 002709; suivant : 002711Isolation and biological activity of frankiamide.
Auteurs : J P Haansuu [Finlande] ; K D Klika ; P P Söderholm ; V V Ovcharenko ; K. Pihlaja ; K K Haahtela ; P M VuorelaSource :
- Journal of industrial microbiology & biotechnology [ 1367-5435 ] ; 2001.
Descripteurs français
- KwdFr :
- Actinomycetales (croissance et développement), Actinomycetales (métabolisme), Amides (composition chimique), Amides (isolement et purification), Amides (pharmacologie), Animaux (MeSH), Bactéries à Gram positif (effets des médicaments et des substances chimiques), Calcium (antagonistes et inhibiteurs), Calcium (métabolisme), Cellules cultivées (MeSH), Champignons (effets des médicaments et des substances chimiques), Composés hétérocycliques (composition chimique), Composés hétérocycliques (isolement et purification), Composés hétérocycliques (pharmacologie), Hypophyse (cytologie), Imides (composition chimique), Imides (isolement et purification), Imides (pharmacologie), Rats (MeSH), Tests de sensibilité microbienne (MeSH).
- MESH :
- antagonistes et inhibiteurs : Calcium.
- composition chimique : Amides, Composés hétérocycliques, Imides.
- croissance et développement : Actinomycetales.
- cytologie : Hypophyse.
- effets des médicaments et des substances chimiques : Bactéries à Gram positif, Champignons.
- isolement et purification : Amides, Composés hétérocycliques, Imides.
- métabolisme : Actinomycetales, Calcium.
- pharmacologie : Amides, Composés hétérocycliques, Imides.
- Animaux, Cellules cultivées, Rats, Tests de sensibilité microbienne.
English descriptors
- KwdEn :
- Actinomycetales (growth & development), Actinomycetales (metabolism), Amides (chemistry), Amides (isolation & purification), Amides (pharmacology), Animals (MeSH), Calcium (antagonists & inhibitors), Calcium (metabolism), Cells, Cultured (MeSH), Fungi (drug effects), Gram-Positive Bacteria (drug effects), Heterocyclic Compounds (chemistry), Heterocyclic Compounds (isolation & purification), Heterocyclic Compounds (pharmacology), Imides (chemistry), Imides (isolation & purification), Imides (pharmacology), Microbial Sensitivity Tests (MeSH), Pituitary Gland (cytology), Rats (MeSH).
- MESH :
- chemical , antagonists & inhibitors : Calcium.
- chemical , chemistry : Amides, Heterocyclic Compounds, Imides.
- cytology : Pituitary Gland.
- drug effects : Fungi, Gram-Positive Bacteria.
- growth & development : Actinomycetales.
- chemical , isolation & purification : Amides, Heterocyclic Compounds, Imides.
- metabolism : Actinomycetales, Calcium.
- chemical , pharmacology : Amides, Heterocyclic Compounds, Imides.
- Animals, Cells, Cultured, Microbial Sensitivity Tests, Rats.
Abstract
An antibiotic produced by the symbiotic actinomycete Frankia strain AiPs1 was isolated from culture broth using optimized thin-layer chromatography and high-performance liquid chromatography (HPLC) methods. The novel compound that was isolated, dubbed frankiamide, displayed antimicrobial activity against all 14 Gram-positive bacterial strains and six pathogenic fungal strains tested. The pathogenic actinomycete Clavibacter michiganensis and the oomycete Phytophthora were especially susceptible. In addition to displaying antimicrobial activity, frankiamide also strongly inhibited 45Ca(2+) fluxes in clonal rat pituitary GH4C1 tumor cells and was comparable to a frequently used calcium antagonist, verapamil hydrochloride. The results of HPLC analysis, supported by both nuclear magnetic resonance and mass spectroscopy studies, showed that frankiamide has a high affinity for Na(+) ions.
DOI: 10.1038/sj.jim.7000164
PubMed: 11598813
Affiliations:
Links toward previous steps (curation, corpus...)
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(drug effects)</term><term>Heterocyclic Compounds (chemistry)</term><term>Heterocyclic Compounds (isolation & purification)</term><term>Heterocyclic Compounds (pharmacology)</term><term>Imides (chemistry)</term><term>Imides (isolation & purification)</term><term>Imides (pharmacology)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Pituitary Gland (cytology)</term><term>Rats (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Actinomycetales (croissance et développement)</term><term>Actinomycetales (métabolisme)</term><term>Amides (composition chimique)</term><term>Amides (isolement et purification)</term><term>Amides (pharmacologie)</term><term>Animaux (MeSH)</term><term>Bactéries à Gram positif (effets des médicaments et des substances chimiques)</term><term>Calcium (antagonistes et inhibiteurs)</term><term>Calcium (métabolisme)</term><term>Cellules cultivées (MeSH)</term><term>Champignons (effets des médicaments et des substances 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xml:lang="en">An antibiotic produced by the symbiotic actinomycete Frankia strain AiPs1 was isolated from culture broth using optimized thin-layer chromatography and high-performance liquid chromatography (HPLC) methods. The novel compound that was isolated, dubbed frankiamide, displayed antimicrobial activity against all 14 Gram-positive bacterial strains and six pathogenic fungal strains tested. The pathogenic actinomycete Clavibacter michiganensis and the oomycete Phytophthora were especially susceptible. In addition to displaying antimicrobial activity, frankiamide also strongly inhibited 45Ca(2+) fluxes in clonal rat pituitary GH4C1 tumor cells and was comparable to a frequently used calcium antagonist, verapamil hydrochloride. The results of HPLC analysis, supported by both nuclear magnetic resonance and mass spectroscopy studies, showed that frankiamide has a high affinity for Na(+) ions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11598813</PMID><DateCompleted><Year>2001</Year><Month>12</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1367-5435</ISSN><JournalIssue CitedMedium="Print"><Volume>27</Volume><Issue>1</Issue><PubDate><Year>2001</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Journal of industrial microbiology & biotechnology</Title><ISOAbbreviation>J Ind Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Isolation and biological activity of frankiamide.</ArticleTitle><Pagination><MedlinePgn>62-6</MedlinePgn></Pagination><Abstract><AbstractText>An antibiotic produced by the symbiotic actinomycete Frankia strain AiPs1 was isolated from culture broth using optimized thin-layer chromatography and high-performance liquid chromatography (HPLC) methods. The novel compound that was isolated, dubbed frankiamide, displayed antimicrobial activity against all 14 Gram-positive bacterial strains and six pathogenic fungal strains tested. The pathogenic actinomycete Clavibacter michiganensis and the oomycete Phytophthora were especially susceptible. In addition to displaying antimicrobial activity, frankiamide also strongly inhibited 45Ca(2+) fluxes in clonal rat pituitary GH4C1 tumor cells and was comparable to a frequently used calcium antagonist, verapamil hydrochloride. The results of HPLC analysis, supported by both nuclear magnetic resonance and mass spectroscopy studies, showed that frankiamide has a high affinity for Na(+) ions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Haansuu</LastName><ForeName>J P</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Division of General Microbiology, Department of Biosciences, University of Helsinki, PO Box 56, Helsinki FIN-00014, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Klika</LastName><ForeName>K D</ForeName><Initials>KD</Initials></Author><Author ValidYN="Y"><LastName>Söderholm</LastName><ForeName>P P</ForeName><Initials>PP</Initials></Author><Author ValidYN="Y"><LastName>Ovcharenko</LastName><ForeName>V V</ForeName><Initials>VV</Initials></Author><Author ValidYN="Y"><LastName>Pihlaja</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Haahtela</LastName><ForeName>K K</ForeName><Initials>KK</Initials></Author><Author ValidYN="Y"><LastName>Vuorela</LastName><ForeName>P M</ForeName><Initials>PM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Ind Microbiol Biotechnol</MedlineTA><NlmUniqueID>9705544</NlmUniqueID><ISSNLinking>1367-5435</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000577">Amides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006571">Heterocyclic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007094">Imides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C431244">frankiamide</NameOfSubstance></Chemical><Chemical><RegistryNumber>SY7Q814VUP</RegistryNumber><NameOfSubstance UI="D002118">Calcium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000192" MajorTopicYN="N">Actinomycetales</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000577" MajorTopicYN="N">Amides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002118" MajorTopicYN="N">Calcium</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006094" MajorTopicYN="N">Gram-Positive Bacteria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006571" MajorTopicYN="N">Heterocyclic Compounds</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007094" MajorTopicYN="N">Imides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010902" MajorTopicYN="N">Pituitary Gland</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2001</Year><Month>02</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2001</Year><Month>06</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>10</Month><Day>13</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>1</Month><Day>5</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>10</Month><Day>13</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11598813</ArticleId><ArticleId IdType="doi">10.1038/sj.jim.7000164</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Finlande</li></country><region><li>Uusimaa</li></region><settlement><li>Helsinki</li></settlement><orgName><li>Université d'Helsinki</li></orgName></list><tree><noCountry><name sortKey="Haahtela, K K" sort="Haahtela, K K" uniqKey="Haahtela K" first="K K" last="Haahtela">K K Haahtela</name><name sortKey="Klika, K D" sort="Klika, K D" uniqKey="Klika K" first="K D" last="Klika">K D Klika</name><name sortKey="Ovcharenko, V V" sort="Ovcharenko, V V" uniqKey="Ovcharenko V" first="V V" last="Ovcharenko">V V Ovcharenko</name><name sortKey="Pihlaja, K" sort="Pihlaja, K" uniqKey="Pihlaja K" first="K" last="Pihlaja">K. Pihlaja</name><name sortKey="Soderholm, P P" sort="Soderholm, P P" uniqKey="Soderholm P" first="P P" last="Söderholm">P P Söderholm</name><name sortKey="Vuorela, P M" sort="Vuorela, P M" uniqKey="Vuorela P" first="P M" last="Vuorela">P M Vuorela</name></noCountry><country name="Finlande"><region name="Uusimaa"><name sortKey="Haansuu, J P" sort="Haansuu, J P" uniqKey="Haansuu J" first="J P" last="Haansuu">J P Haansuu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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